Homer 1/2/3 (N-Term) 抗体
适用: 人, 小鼠, 大鼠, 斑马鱼
ICC, WB
宿主: 兔
Polyclonal
unconjugated
(2 references)-
- 抗原
- Homer 1/2/3
- 抗原表位
- N-Term
- 适用
- 人, 小鼠, 大鼠, 斑马鱼
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunocytochemistry (ICC), Western Blotting (WB)
- 特异性
- The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Purified recombinant N-terminal parts of Homer 1, 2 and 3.
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- 应用备注
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
ELISA: not tested yet - 说明
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ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).
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: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
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- 抗原
- Homer 1/2/3
- 背景
- Synonyms: VesL 1, Syn47
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