CACNA1B 抗体 (AA 1921-2222)
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- 抗原 See all CACNA1B 抗体
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
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抗原表位
- AA 1921-2222
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适用
- 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CACNA1B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Specific for Ca2+ channel alpha-1E.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).
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- Discover our top product CACNA1B Primary Antibody
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- 应用备注
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WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 - 说明
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WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- 抗原
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
- 别名
- Ca2+ Channel (CACNA1B 产品)
- 别名
- BIII antibody, CACNL1A5 antibody, CACNN antibody, Cav2.2 antibody, AW050276 antibody, AW060892 antibody, AW822256 antibody, Cchn1a antibody, alpha(1B) antibody, calcium voltage-gated channel subunit alpha1 B antibody, calcium channel, voltage-dependent, N type, alpha 1B subunit antibody, CACNA1B antibody, Cacna1b antibody
- 背景
- Synonyms: Cav2.3
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