Uncoating ATPase (AA 391-546) 抗体
适用: Cow, Hamster, 人, 小鼠, 大鼠
ICC, IHC, WB
宿主: 小鼠
Monoclonal
3C5
unconjugated
(12 references)-
- 抗原
- Uncoating ATPase
- 抗原表位
- AA 391-546
- 适用
- Cow, Hamster, 人, 小鼠, 大鼠
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Specific for uncoating ATPase.
- 纯化方法
- purified IgG
- 免疫原
- Purified bovine ATPase. Epitope Between aa 391-546.
- 克隆位点
- 3C5
- 亚型
- IgG1
-
- 应用备注
-
WB: 1 : 500 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC-P: 1 : 200 - 说明
-
For fixation a 20 min treatment with 3.7 % PFA in PBS is recommended. When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
-
-
: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).
: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
: "Synaptic function of nicastrin in hippocampal neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 24, pp. 8973-8, (2014) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).
: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).
: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).
: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).
: "Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 607-20, (2001) (PubMed).
: "ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70." in: Molecular biology of the cell, Vol. 9, Issue 8, pp. 2217-29, (1998) (PubMed).
: "Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70)." in: Journal of cell science, Vol. 107 ( Pt 5), pp. 1185-96, (1994) (PubMed).
-
: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).
-
- 抗原
- Uncoating ATPase
- 背景
- Synonyms: hsc 70
-
You are here:
