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Alpha, beta Synuclein (AA 2-25), (N-Term) 抗体

适用: 小鼠, 大鼠, 斑马鱼 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1742282
发货至: 中国
  • 抗原
    Alpha, beta Synuclein
    抗原表位
    • 15
    • 1
    • 1
    AA 2-25, N-Term
    适用
    • 25
    • 24
    • 24
    • 15
    小鼠, 大鼠, 斑马鱼
    宿主
    • 22
    • 4
    克隆类型
    • 16
    • 10
    多克隆
    标记
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    非结合性
    应用范围
    • 14
    • 14
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    特异性
    Recognizes alpha synuclein and beta synuclein.
    纯化方法
    antiserum
    免疫原
    Synthetic peptide DVFMKGLSKAKEGVVAAAEKTKQG (aa 2-25 in alpha synuclein), coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
  • 应用备注
    WB: 1 : 1000 (AP staining)
    ICC: 1 : 500
    IHC: 1 : 500
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PBS
    注意事项
    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
    储存条件
    4 °C/-20 °C
    储存方法
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Siebert, Kahle, Kramer, Isik, Schlüter, Schulz-Schaeffer, Brück: "Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain." in: Journal of neurochemistry, Vol. 114, Issue 4, pp. 1007-18, (2010) (PubMed).

    Tong, Wong, Guttman, Ang, Forno, Shimadzu, Rajput, Muenter, Kish, Hornykiewicz, Furukawa: "Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation." in: Brain : a journal of neurology, Vol. 133, Issue Pt 1, pp. 172-88, (2010) (PubMed).

    Cole, Dieuliis, Leo, Mitchell, Nussbaum: "Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification." in: Experimental cell research, Vol. 314, Issue 10, pp. 2076-89, (2008) (PubMed).

    Colapinto, Mila, Giraudo, Stefanazzi, Molteni, Rossetti, Bergamasco, Lopiano, Fasano: "alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity." in: Biochemical and biophysical research communications, Vol. 349, Issue 4, pp. 1294-300, (2006) (PubMed).

    Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

    Steinacker, Schwarz, Reim, Brechlin, Jahn, Kratzin, Aitken, Wiltfang, Aguzzi, Bahn, Baxter, Brose, Otto: "Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein." in: Molecular and cellular biology, Vol. 25, Issue 4, pp. 1339-46, (2005) (PubMed).

  • 抗原
    Alpha, beta Synuclein
    别名
    alpha/beta Synuclein
    背景
    Synonyms: alpha-Synuclein, beta-Synuclein
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