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UNC13A/Munc13-1 抗体 (AA 3-317)

UNC13A 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IP, ICC, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1742277
发货至: 中国
  • 抗原 See all UNC13A/Munc13-1 (UNC13A) 抗体
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    抗原表位
    • 2
    • 2
    • 1
    AA 3-317
    适用
    人, 小鼠, 大鼠, 斑马鱼
    宿主
    • 3
    • 3
    • 1
    • 1
    克隆类型
    • 7
    • 1
    多克隆
    标记
    • 8
    This UNC13A/Munc13-1 antibody is un-conjugated
    应用范围
    • 8
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    特异性
    Specific for munc 13-1.
    纯化方法
    antiserum
    免疫原
    Recombinant rat munc 13-1 (aa 3-317).
  • 应用备注
    WB: 1 : 1000 (AP staining)
    ICC: 1 : 500
    IHC: 1 : 200
    ELISA: not tested yet
    说明

    IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
    缓冲液
    PBS
    注意事项
    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
    储存条件
    4 °C/-20 °C
    储存方法
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

    Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

    Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

  • 抗原
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    别名
    Munc 13-1 (UNC13A 产品)
    别名
    Munc13-1 antibody, Unc13h1 antibody, 2410078G03Rik antibody, unc-13 homolog A antibody, unc-13 homolog A (C. elegans) antibody, Unc13a antibody, UNC13A antibody
    途径
    Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
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