Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) 抗体
适用: 大鼠
ICC, WB
宿主: 小鼠
Monoclonal
151-2
unconjugated
(3 references)-
- 抗原
- Syntaxin 12/13
- 抗原表位
- AA 1-250, Cytoplasmic Domain
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适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
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应用范围
- Immunocytochemistry (ICC), Western Blotting (WB)
- 特异性
- Specific for syntaxin 12/13.
- 纯化方法
- purified IgG
- 免疫原
- Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1-250).
- 克隆位点
- 151-2
- 亚型
- IgG1
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anti-Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) antibody
适用: 大鼠, 小鸡, Hamster, 人, 小鼠 ICC, WB, IP, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
-
WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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-
: "Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation." in: Cell reports, (2015) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 595-610, (2013) (PubMed).
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: "Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation." in: Cell reports, (2015) (PubMed).
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- 抗原
- Syntaxin 12/13
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