Proton ATPase (AA 826-838) 抗体
适用: Hamster, 小鼠, 大鼠
WB, ICC, IHC
宿主: 兔
Polyclonal
unconjugated
(2 references)-
- 抗原
- Proton ATPase
- 抗原表位
- AA 826-838
- 适用
- Hamster, 小鼠, 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- 特异性
- Specific for the alpha1 116kDa subunit.
- 纯化方法
- antiserum
- 免疫原
- Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
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- 应用备注
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC-P: 1 : 100 - 说明
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WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS
- 注意事项
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, (2014) (PubMed).
: "Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice." in: PLoS ONE, Vol. 8, Issue 7, pp. e68215, (2013) (PubMed).
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: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, (2014) (PubMed).
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- 抗原
- Proton ATPase
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