VAMP1, 2, 3 (AA 1-81) 抗体
(1 reference)-
- 抗原
- VAMP1, 2, 3
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抗原表位
- AA 1-81
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适用
- 小鼠, 大鼠, 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- Specific for all three isoforms VAMP 1, 2 and 3.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1-81).
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anti-VAMP1, 2, 3 (AA 1-81) antibody
适用: 小鼠, 大鼠, 人 WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-VAMP1, 2, 3 (AA 1-81) antibody适用: 小鼠, 大鼠 WB, ICC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
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WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 200 - 说明
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ICC: Methanol and acetone fixation, see Annaert et al., 1997 This antibody is recommended for ICC of non-neuronal cells. This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." in: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, (2014) (PubMed).
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: "Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." in: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, (2014) (PubMed).
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- 抗原
- VAMP1, 2, 3
- 背景
- Synonyms: VAMP 3
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