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Nibrin 抗体 (AA 467-615)

Name: Nibrin 抗体 (AA 467-615)
Brand: antibodies-online
SKU: ABIN1724942
Availability: OutOfStock

NBN 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 7E4C2 unconjugated

Western blot analysis using NBN mouse mAb against Jurkat (1) cell lysate. (Nibrin 抗体 (AA 467-615))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Nibrin 抗体 (AA 467-615))

ELISA

Flow cytometric analysis of Hela cells using NBN mouse mAb (green) and negative control (red). (Nibrin 抗体 (AA 467-615))

FACS

Immunofluorescence analysis of Hela cells using NBN mouse mAb (green). (Nibrin 抗体 (AA 467-615))

Western blot analysis using NBN mAb against human NBN (AA: 467-615) recombinant protein. (Nibrin 抗体 (AA 467-615))

Western blot analysis using NBN mAb against HEK293 (1) and NBN (AA: 467-615)-hIgGFc transfected HEK293 (2) cell lysate. (Nibrin 抗体 (AA 467-615))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NBN mouse mAb with DAB staining. (Nibrin 抗体 (AA 467-615))

IHC

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using NBN mouse mAb with DAB staining. (Nibrin 抗体 (AA 467-615))

IHC

8 images

(2 references)

产品编号 ABIN1724942

发货至: 中国

Datasheet as PDF

抗原 See all Nibrin (NBN) 抗体

Nibrin (NBN)
抗原表位
38

14

8

8

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 467-615
适用
83

57

32

2

1

1

1

1
人
宿主
101

13
小鼠
Compatible Secondaries
克隆类型
89

25
单克隆
标记
59

6

5

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Nibrin antibody is un-conjugated
应用范围
92

37

28

25

25

25

20

14

12

10

6

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

原理

NBN Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human NBN (AA: 467-615) expressed in E. Coli.

克隆位点

7E4C2

亚型

IgG2a
anti-Nibrin (NBN) (AA 461-754) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A2 unconjugated

anti-Nibrin (NBN) (AA 461-754) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A9 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 8E7 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 6E11 unconjugated

anti-Nibrin (NBN) (AA 435-754) antibody
NBN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Nibrin (NBN) (AA 645-754) antibody
NBN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E4 unconjugated

anti-Nibrin (NBN) antibody
KO Validated NBN 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Nibrin (NBN) (AA 714-745), (C-Term) antibody
NBN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nibrin (NBN) (pSer343) antibody
NBN 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Zuhlke, Johnson, Okoth, Stoffel, Robbins, Tembe, Salinas, Zheng, Xu, Carpten, Lange, Isaacs, Cooney: "Identification of a novel NBN truncating mutation in a family with hereditary prostate cancer." in: Familial cancer, Vol. 11, Issue 4, pp. 595-600, (2012) (PubMed).

Zheng, Zhang, Jiang, You, Liu, Lu, Zhou: "Functional NBS1 polymorphism is associated with occurrence and advanced disease status of nasopharyngeal carcinoma." in: Molecular carcinogenesis, Vol. 50, Issue 9, pp. 689-96, (2011) (PubMed).
抗原

Nibrin (NBN)

别名

NBN (NBN 产品)

背景

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

分子量

85 kDa

基因ID

4683

UniProt

O60934

途径

DNA Damage Repair, Production of Molecular Mediator of Immune Response