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PCNA 抗体 (AA 53-196)

Name: PCNA 抗体 (AA 53-196)
Brand: antibodies-online
SKU: ABIN1724760
Availability: OutOfStock

Cited in 2+ publications. This 小鼠单克隆 anti-PCNA antibody (Clone 7H4F8) specifically detects PCNA in WB, IHC, ELISA 和 FACS. The antibody is reactive with 人, 小鼠和大鼠 samples.

Western blot analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate. (PCNA 抗体 (AA 53-196))

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA mouse mAb with DAB staining. (PCNA 抗体 (AA 53-196))

IHC

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PCNA 抗体 (AA 53-196))

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate. (PCNA 抗体 (AA 53-196))

Western blot analysis using PCNA mAb against human PCNA recombinant protein. (PCNA 抗体 (AA 53-196))

Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple). (PCNA 抗体 (AA 53-196))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA mouse mAb with DAB staining. (PCNA 抗体 (AA 53-196))

IHC

7 images

(2 references)

产品编号 ABIN1724760

发货至: 中国

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Quick Overview for PCNA 抗体 (AA 53-196) (ABIN1724760)

抗原

See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This PCNA antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

克隆位点

7H4F8

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AA 53-196

原理

PCNA Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

亚型

IgG1
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP, IHC (p), ICC, IHC (fro), FISH 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibody
PCNA 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17486 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (C-Term) antibody
PCNA 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Takawa, Cho, Hayami, Toyokawa, Kogure, Yamane, Iwai, Maejima, Ueda, Masuda, Dohmae, Field, Tsunoda, Kobayashi, Akasu, Sugiyama, Ohnuma, Atomi, Ponder, Nakamura, Hamamoto: "Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression." in: Cancer research, Vol. 72, Issue 13, pp. 3217-27, (2012) (PubMed).

Zhao, Ho, Lo, Espejo, Bedford, Hung, Wang: "Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer." in: PLoS ONE, Vol. 7, Issue 1, pp. e29416, (2012) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA

背景

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

分子量

28.7 kDa

基因ID

5111

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy