Arp2/Arp3 (AA 301-394) 抗体 (HRP)
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- 抗原
- Arp2/Arp3
- 抗原表位
- AA 301-394
- 适用
- 小鼠, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
- 交叉反应
- 小鼠, 非洲爪蟾
- 预测反应
- Human,Rat,Cow,Pig,Chicken
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human ARP2/ARP3
- 亚型
- IgG
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- 应用备注
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Arp2/Arp3
- 背景
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Synonyms: Actin like protein 2, Actin related protein 2, Actin-like protein 2, ACTIN-RELATED PROTEIN 2, ACTR2, ARP2 actin related protein 2 homolog yeast, ARP2, ARP2/3 COMPLEX, ARP2_HUMAN, OTTHUMP00000159937, OTTHUMP00000202270.
Background: Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.
- 基因ID
- 10097
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