ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
交叉反应 (详细)
Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
纯度
Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].
内毒素水平
Low Endotoxin : No
免疫原
Albumin (Bovine Serum) Immunogen Type: Native Protein
Anti-BOVINE ALBUMIN (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. It is also used as a nutrient in cell and microbial culture. Specific conditions should be optimized by user. ELISA Dilution: 1:20.000 - 1:80.000 Immunohistochemistry Dilution: 1:500 - 1:2.500 Western Blot Dilution: 1:2.000 - 1:10.000
限制
仅限研究用
状态
Liquid
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
注意事项
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Do NOT add Sodium Azide!
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
有效期
Expiration date is one (1) year from date of opening.