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- 抗原 See all Alkaline Phosphatase (ALP) 抗体
- Alkaline Phosphatase (ALP)
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Alkaline Phosphatase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product ALP Primary Antibody
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anti-Alkaline Phosphatase (ALP) (AA 56-83) antibody
ALP 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人, Cow IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 282-309) antibodyALP 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 21-35) antibodyALP 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Alkaline Phosphatase has been assayed against 1.0 µg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Alkaline Phosphatase (ALP)
- 别名
- Alkaline Phosphatase (ALP 产品)
- 别名
- ALP antibody, ALP1 antibody, CRASH antibody, ECK0378 antibody, JW0374 antibody, psiA antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, ILC antibody, CTACK antibody, ESkine antibody, Akp2 antibody, alp antibody, zgc:56672 antibody, DDBDRAFT_0205491 antibody, DDBDRAFT_0231570 antibody, DDB_0205491 antibody, DDB_0231570 antibody, Actn2lp antibody, Alp antibody, asparaginase like 1 antibody, bacterial alkaline phosphatase antibody, C-C motif chemokine ligand 27 antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase antibody, PDZ and LIM domain 3 antibody, ASRGL1 antibody, phoA antibody, Asrgl1 antibody, CCL27 antibody, alpl antibody, alp antibody, Pdlim3 antibody
- 背景
- At a high pH, Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research. Synonyms: Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- 基因ID
- 280993
- UniProt
- P19111
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