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- 抗原 See all GLN1 抗体
- GLN1 (Glutamine Synthetase (GLN1))
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适用
- Brevibacterium
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GLN1 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- 交叉反应 （详细）
- Cross reactivity against Glutamine Synthetase from other sources is unknown.
- 纯度
- Anti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Glutamine Synthetase [Microbial]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product GLN1 Primary Antibody
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anti-Glutamine Synthetase (GLN1) (AA 1-373) antibody
GLN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 285-373) antibodyGLN1 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 50-250) antibodyGLN1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-750M unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 300-350) antibodyGLN1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) antibody (HRP)GLN1 适用: Bacteria WB, IP, ELISA 宿主: 山羊 Polyclonal HRP
anti-Glutamine Synthetase (GLN1) antibody (Biotin)GLN1 适用: Brevibacterium WB, IP, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Glutamine Synthetase (GLN1) antibodyGLN1 适用: Bacteria ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) antibodyGLN1 适用: 小鼠, 大鼠 WB, IHC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
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Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 µg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- GLN1 (Glutamine Synthetase (GLN1))
- 别名
- Glutamine Synthetase (GLN1 产品)
- 别名
- GLNS antibody, GS antibody, PIG43 antibody, PIG59 antibody, Glns antibody, glutamate-ammonia ligase antibody, glutamate-ammonia ligase (glutamine synthetase) antibody, glutamine synthetase antibody, GLUL antibody, Glul antibody, GLN1 antibody
- 背景
- Glutamine Synthetase is a key enzyme in the metabolism of nitrogen. Glutamine synthetase catalyzes an ATP-dependent condensation reaction between ammonia and glutamate to yield glutamine. Glutamine is a key builder of proteins as well as a vehicle to deliver nitrogen atoms to enzymes that build molecules dependent on nitrogen. Glutamine Synthetase from a microbial source is composed of twelve subunits that each house an active site. During the reaction of glutamine synthetase, the active sites bind ammonia and glutamate, as well as an ATP molecule to power the reaction. Negative feedback regulation is provided by the active sites ability to weakly bind other molecules and once their concentrations rise too high, the enzyme shuts off. Glutamine Synthetase has applications in neuroscience due to location in astrocytes within the brain and fluctuations in glutamine synthetase can detrimentally effect the astrocytes. Anti-Glutamine Synthetase (Microbial) Antibody is ideal for investigators in Molecular Biology, Neuroscience, and Enzymology. Synonyms: Glutamine synthetase EC=6.3.1.2
- 基因ID
- 3345165
- UniProt
- Q79VE3
- 途径
- Positive Regulation of Peptide Hormone Secretion
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