Nop5p 抗体
适用: Saccharomyces cerevisiae
ICC, IF, IHC, WB
宿主: 小鼠
Monoclonal
34B12
unconjugated
1 image
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- 抗原
- Nop5p
- 适用
- Saccharomyces cerevisiae
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- conc. tissue culture supernatant
- 过滤
- Sterile filtered
- 克隆位点
- 34B12
- 亚型
- IgG3
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- 应用备注
- For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µL for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/mL.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4°C short term or -20°C long term.
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- 抗原
- Nop5p
- 背景
- Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).
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