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- 抗原 See all Histone H2B 抗体
- Histone H2B
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抗原表位
- Lys20
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone H2B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.
- 交叉反应 （详细）
- Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.
- 纯化方法
- Immunoaffinity chromatography using acetylpeptide, cross-adsorbed with the corresponding non-acetylpeptide
- 免疫原
- a KLH-coupled synthetic acetylpeptide corresponding - AA surrounding Lys20 of human Histone H2B. .
- 亚型
- IgG
- Top Product
- Discover our top product Histone H2B Primary Antibody
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anti-Histone H2B (acLys15) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys12) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys12) antibody适用: 人 WB, ELISA, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys15) antibody适用: 人 WB, ELISA, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys12) antibody适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人 WB, IHC, IF, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys20) antibody适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (meLys5) antibody适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人 WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLOther Applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- Histone H2B
- Abstract
- Histone H2B 产品
- 别名
- Hist1h2bc antibody, H2B antibody, HISTONE H2B antibody, MRN17.11 antibody, MRN17_11 antibody, histone B2 antibody, H2B1 antibody, HIS2B antibody, histone cluster 1 H2B family member k antibody, histone H2B antibody, histone B2 antibody, histone H2B.3 antibody, Hist1h2bk antibody, H2B antibody, HTB2 antibody, THAPSDRAFT_27352 antibody, THAPSDRAFT_37309 antibody, THAPS_6947 antibody, THAPSDRAFT_34210 antibody, LOC426037 antibody, LOC107802329 antibody
- 背景
- Histone H2B is a core component of nucleosome that facilitates nucleosome wrapping and DNA compacting into chromatin. This limits the accessibility of DNA to the cellular machineries for which DNA is required as a template. Histones thereby play a central role in transcription regulation, DNA repair and replication as well as chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, of which the process is called the histone code, and nucleosome remodeling.Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic acetylpeptide corresponding to residues surrounding Lys20 of human Histone H2B. (Swiss Prot: Q5QNW6).
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