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Vimentin 抗体 (AA 400-500)

Name: Vimentin 抗体 (AA 400-500)
Brand: antibodies-online
SKU: ABIN1574034
Availability: OutOfStock

VIM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

Western blot analysis of cell lysates using 1 µg/mL Rabbit Anti-Vimentin Polyclonal Antibody (ABIN398721) Lane 1, 3: Hela cell lysateLane 2, 4: HEK293 cell lysate Primary antibody: Lane 1, 2: Rabbit Anti-Vimentin Polyclonal AntibodyLane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptideThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. (Vimentin 抗体 (AA 400-500))

Immunohistochemistry analysis of human spleen tissue slide (Paraffin embedded) using Rabbit Anti-Vimentin Polyclonal Antibody (Left, ABIN398721) and Purified Rabbit IgG (Whole molecule) Control (Right, ABIN398653) (Vimentin 抗体 (AA 400-500))

IHC

Flow cytometric analysis of Ramos cells using Vimentin Antibody, pAb, Rabbit (ABIN398721, shaded histogram) or with an isotype control antibody (ABIN398653, open histogram), followed by R-PE conjugated anti-rabbit IgG. (Vimentin 抗体 (AA 400-500))

FACS

3 images

产品编号 ABIN1574034

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 400-500
适用
332

125

124

61

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
189

129

9

6

4

4

2
兔
Compatible Secondaries
克隆类型
197

145
多克隆
标记
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
281

131

99

97

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

特异性

Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

交叉反应（详细）

Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

纯化方法

Immunoaffinity chromatography

免疫原

Synthetic peptide (KLH-coupled) derived from within AA 400-500 of human vimentin

亚型

IgG

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VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

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VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLFlow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).

途径

Caspase Cascade in Apoptosis