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Vimentin 抗体 (AA 70-120)

Name: Vimentin 抗体 (AA 70-120)
Brand: antibodies-online
SKU: ABIN1574032
Availability: OutOfStock

VIM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Hela and NIH/3T3 cell lysates using 1 µg/mL Rabbit Anti-Vimentin Polyclonal Antibody (ABIN398717) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG (Vimentin 抗体 (AA 70-120))

Western blot analysis of HEK293 cell lysate using 1 µg/mL Rabbit Anti-Vimentin Polyclonal Antibody (ABIN398717) Primary Antibody: Lane 1: Rabbit Anti-Vimentin Polyclonal AntibodyLane 2: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptideThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG (Vimentin 抗体 (AA 70-120))

Immunohistochemistry analysis of human spleen tissue slide (Paraffin embedded) usingRabbit Anti-Vimentin Polyclonal Antibody (ABIN398717) (Vimentin 抗体 (AA 70-120))

IHC

3 images

产品编号 ABIN1574032

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

16

12

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 70-120
适用
324

124

123

60

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
186

123

9

6

4

4

2
兔
Compatible Secondaries
克隆类型
197

136
多克隆
标记
191

19

13

13

10

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
273

127

97

94

94

59

46

40

39

28

23

18

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

特异性

Rabbit Anti-Vimentin Polyclonal Antibody detects endogenous levels of human and mouse vimentin protein. Sequence homology predicts that it will also react with rat vimentin protein.

交叉反应（详细）

Rabbit Anti-Vimentin Polyclonal Antibody detects endogenous levels of human and mouse vimentin protein. Sequence homology predicts that it will also react with rat vimentin protein.

纯化方法

Immunoaffinity chromatography

免疫原

Synthetic peptide (KLH-coupled) within AA 70-120 of human vimentin

亚型

IgG

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VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

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VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure.Rabbit Anti-Vimentin Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 70-120 of human vimentin protein (Swiss Prot: P08670).

途径

Caspase Cascade in Apoptosis