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CNTF 抗体

CNTF 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1497554
发货至: 中国
  • 抗原 See all CNTF 抗体
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    适用
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    多克隆
    标记
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    This CNTF antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human CNTF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human CNTF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product CNTF Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: LS-B8208 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human CNTF (1 μg/mL) a concentration of 7-11 μg/mL of this antibody is required. ELISA: To detect hCNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CNTF (LS-C104338) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    别名
    CNTF (CNTF 产品)
    别名
    GPA antibody, CNTF antibody, HCNTF antibody, AI429687 antibody, ciliary neurotrophic factor antibody, CNTF antibody, Cntf antibody
    背景
    Name/Gene ID: CNTF

    Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF
    基因ID
    1270
    UniProt
    P26441
    途径
    JAK/STAT Signaling
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