(1 reference)-
- 抗原
- mLC-2
-
抗原表位
- AA 5-54, pThr17
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
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应用范围
- ELISA, Immunohistochemistry (IHC)
- 特异性
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MLC-2 (Phospho-Thr17+Ser18) Antibody detects endogenous levels of MLC-2 only when phosphorylated at Thr17 and Ser18.
PhosphorylationH:T17+S18, M:T19+S20, R:T18+S19
- 纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
- 免疫原
- The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18.
- 亚型
- IgG
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-
- 应用备注
- IHC: 1:50~1:100 ELISA: 1:10000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Stable at -20°C for at least 1 year.
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: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).
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: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).
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- 抗原
- mLC-2
- 别名
- 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, Mrlc2 antibody, Mrlcb antibody, Mylc2b antibody, CMH10 antibody, MLC2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin light chain 12B antibody, myosin light chain 2 antibody, Mylpf antibody, Myl12b antibody, MYL2 antibody
- 背景
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Synonyms: MLCB, MLRA, MLRM, MRCL3, Myosin RLC, Myosin RLC-A, Myosin regulatory light chain, Myosin regulatory light chain 2, nonsarcomeric, Myosin
NCBI Gene Symbol: MYL12A
- 分子量
- 19 kDa
- 基因ID
- 10627
- OMIM
- 609211
- UniProt
- P19105, O14950, P24844
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