SPARC 抗体
Quick Overview for SPARC 抗体 (ABIN1450173)
抗原
See all SPARC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin. SPARC produced by various cells grown in culture. Cross reacts with Rat Osteonectin.Aff. Const.:5 x 10e-9 M
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纯化方法
- Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.
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免疫原
- Crude bone extract containing native Osteonectin.
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亚型
- IgG1
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anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 101-200) antibody
SPARC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibodySPARC 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibodySPARC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19869 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibodySPARC 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibodySPARC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 10.8 mg/mL (OD280 nm, E1% = 14)
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缓冲液
- 50 % Glycerol/H2O (vol/vol), None
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C/-80 °C
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储存方法
- Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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别名
- SPARC / Osteonectin
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背景
- SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine
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基因ID
- 6678
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NCBI登录号
- NP_003109
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UniProt
- P09486
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途径
- Autophagy
抗原
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