RPA1 抗体 (AA 201-300) (Cy3)
Quick Overview for RPA1 抗体 (AA 201-300) (Cy3) (ABIN1407951)
抗原
See all RPA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 201-300
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交叉反应
- 人
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预测反应
- Mouse,Rat,Dog,Pig,Rabbit
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human RPA70
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亚型
- IgG
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anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (C-Term) antibodyRPA1 适用: 人 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (Internal Region) antibodyRPA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 小鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-240) antibodyRPA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- RPA1 (Replication Protein A1, 70kDa (RPA1))
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别名
- RPA70
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背景
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Synonyms: Dmrpa1, Drosophila Replication Protein A, DRPA, HSSB, Human single stranded DNA binding protein, MST075, MSTP075, p70 antibody REPA1, Replication factor A, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit, Replication protein A 70 kDa DNA binding subunit, Replication protein A1 70 kDa, Replication protein A1, RF A, RF-A protein 1, RFA, RFA1_HUMAN, RP A, RP-A p70, RPA 70, RPA, RPA1, Single stranded binding protein 70, Single-stranded DNA-binding protein.
Background: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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