PDZRN3 抗体 (Alexa Fluor 350)
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- 抗原 See all PDZRN3 抗体
- PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDZRN3 antibody is conjugated to Alexa Fluor 350
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应用范围
- Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3
- 亚型
- IgG
- Top Product
- Discover our top product PDZRN3 Primary Antibody
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anti-PDZ Domain Containing Ring Finger 3 (PDZRN3) (AA 133-404) antibody
PDZRN3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-PDZ Domain Containing Ring Finger 3 (PDZRN3) (AA 1-1066) antibodyPDZRN3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- IF(IHC-P) 1:50-200
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PDZRN3 (PDZ Domain Containing Ring Finger 3 (PDZRN3))
- 别名
- SEMCAP3 (PDZRN3 产品)
- 别名
- MGC80196 antibody, pdzrn3 antibody, LNX3 antibody, SEMACAP3 antibody, 1110020C07Rik antibody, AI429718 antibody, AL023082 antibody, Semcap3 antibody, PDZ domain containing ring finger 3 antibody, PDZ domain containing ring finger 3 L homeolog antibody, PDZ domain containing RING finger 3b antibody, E3 ubiquitin-protein ligase PDZRN3 antibody, PDZ domain containing RING finger 3 antibody, PDZRN3 antibody, pdzrn3.L antibody, pdzrn3b antibody, LOC100228989 antibody, pdzrn3 antibody, Pdzrn3 antibody
- 背景
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Synonyms: Ligand of Numb protein X 3, likely ortholog of mouse semaF cytoplasmic domain associated protein 3, LNX3, PDZ domain containing ring finger 3, Protein SEMACAP3, SEMACAP3, Semaphorin cytoplasmic domain associated protein, PZRN3_HUMAN.
Background: PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin.
- 基因ID
- 23024
- 途径
- Skeletal Muscle Fiber Development
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