CLEC14A 抗体 (AA 260-310)
(1 validation)-
- 抗原 See all CLEC14A 抗体
- CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))
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抗原表位
- AA 260-310
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CLEC14A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A
- 亚型
- IgG
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anti-C-Type Lectin Domain Family 14, Member A (CLEC14A) (AA 1-490) antibody
CLEC14A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-C-Type Lectin Domain Family 14, Member A (CLEC14A) (AA 278-349) antibodyCLEC14A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100004
- 日期
- 2015.11.23
- 抗原
- CLEC14A
- Lot Number
- Method validated
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
- Positive Control
- Tumor cell transplanted mouse brain was dissected
- Negative Control
- Notes
- Primary Antibody
- ABIN1385496
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30 min (or RT for 30min)
- Wash three times with PBS 5min (shake gently)
- Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
- Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
- Rabbit-anti-CLEC14A antibody (ABIN1385496) diluted 1:50-1:400
- Wash three times with PBS 5min (shake gently)
- Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
- Donkey-anti-Rabbit-Cy3
- DAPI 1:100
- Wash three times with PBS 5min (shake gently)
- Add three drops of water-based Immu-mount
- Cover with 24x60 cover glass
- Imaging
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN1385496 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
生效 #100004 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of CLEC14A antibody ABIN1385496 on mouse brain tumor sections. A. ABIN1385496 diluted 1:100 (blue), DAPI counterstaining (red). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.]()
IF validation of CLEC14A antibody ABIN1385496 on mouse brain tumor sections. A. ABIN1385496 diluted 1:100 (blue), DAPI counterstaining (red). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))
- 别名
- EGFR5 (CLEC14A 产品)
- 别名
- C14orf27 antibody, CEG1 antibody, EGFR-5 antibody, 1200003C23Rik antibody, AI642649 antibody, C-type lectin domain containing 14A antibody, C-type lectin domain family 14, member a antibody, CLEC14A antibody, Clec14a antibody
- 背景
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Synonyms: CEG1, EGFR-5, C14orf27, C-type lectin domain family 14 member A, Epidermal growth factor receptor 5, CLEC14A, EGFR5, UNQ236/PRO269
Background: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting.
- 基因ID
- 161198
- UniProt
- Q86T13
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