Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.
This antibody is suitable for ELISA and Western Blotting procedures. For direct ELISA, a 1:66,000 dilution detects Botulinum HccB and a 1:42,000 dilutions detects Clostridium botulinum Type B (BTB). For Western blot, the recommended dilution is between 1:1000 and 1:2000. Optimal working dilutions should be determined for your particular assay conditions.
限制
仅限研究用
状态
Liquid
浓度
2.0 mg/ml
缓冲液
Phosphate Buffered Saline (PBS), pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
Jenko, Zhang, Kostenko, Fan, Garcia-Rodriguez, Lou, Marks, Varnum: "Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins." in: The Analyst, Vol. 139, Issue 20, pp. 5093-102, (2015) (PubMed).