Dendritic Cells 抗体 (FITC)
适用: 大鼠
FACS
宿主: 小鼠
Monoclonal
OX-62
FITC
-
- 抗原
- Dendritic Cells
-
适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- FITC
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- Rat lymphoid and non-lymphoid dendritic cells
- 纯化方法
- Purified from ascites via Protein G Chromatography
- 克隆位点
- OX-62
- 亚型
- IgG1
-
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- 0.1 mg/ml
- 缓冲液
- FITC conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- Dendritic Cells
- 别名
- Dendritic Cell
- 背景
- ABIN133165 (OX-62) monoclonal antibody was raised against density gradient-enriched rat veiled (dendritic) cells obtained from lymph. This antibody only labeled cells characteristic of veiled cells the antigen recognized by ABIN133165 is expressed at low to moderate levels by a subset of spleen, thymus and bone marrow dendritic cells. In lymphoid tissues, the labeling correlated with dendritic cells (DC), however in skin, MHC Class II cells were ABIN133165 , while other CD3 cells with dendritic morphology were strongly stained with ABIN133165. Therefore, it seems that this antibody may be restricted to DC and probably T cells. (This has since been contradicted by the fact that the CD3 cells with dendritic morphology were confirmed to stain negative for ABIN133204 (OX-19), and are therefore not T cells.) The ABIN133165 antigen has the biochemical characteristics of an integrin (ABIN133165 recognizes the -like subunit) as determined by SDS-PAGE. This monoclonal antibody is useful for cell purification, delineating lineage relationships among cells with dendritic morphology and for molecular studies. The OX-62 antigen is not essential for T cell activation. The ABIN133165 clone has been use in flow cytometry, immunohistochemistry, immunoprecipitation and cell surface radioiodination.
-
You are here:
