Concentration Definition: by UV absorbance at 280 nm
免疫原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.
PCSK2
适用: 小鼠, 大鼠
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC
宿主: 兔
Polyclonal
unconjugated
应用备注
This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates. The antibody is also functional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.
限制
仅限研究用
状态
Liquid
浓度
1.36 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
抗原
PCSK2
(Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2))
PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2. Synonyms: E3 SUMO-protein ligase CBX4 Chromobox protein homolog 4 Polycomb 2 homolog Pc2 hPc2