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IRS1 抗体 (pSer307)

Name: IRS1 抗体 (pSer307)
SKU: ABIN129580
Availability: OutOfStock

This anti-IRS1 antibody is a 兔多克隆 antibody detecting IRS1 in WB, IHC 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 1 publication.

Western blot using Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead). (IRS1 抗体 (pSer307))

1 image

(1 reference)

产品编号 ABIN129580

Supplier Product No.: 600-401-445

发货至: 中国

Datasheet as PDF

Quick Overview for IRS1 抗体 (pSer307) (ABIN129580)

抗原

See all IRS1 抗体

IRS1 (Insulin Receptor Substrate 1 (IRS1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This IRS1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Discover our top product IRS1 Primary Antibody

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pSer307

Supplier Product No.

600-401-445

Supplier

Rockland

原理

IRS1 phospho S307 Antibody

交叉反应（详细）

This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue.

产品特性

Synonyms: rabbit anti-IRS1 pS307 antibody, IRS-1, IRS 1, HIRS 1 antibody, HIRS-1 antibody, Insulin Receptor Substrate 1 antibody

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.
Immunogen Type: Conjugated Peptide

亚型

IgG
anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (Ser312) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 274-323), (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠, 山羊 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.
Western Blot Dilution: 1:250 - 1:1,500
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

0.76 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, (2006) (PubMed).
抗原

IRS1 (Insulin Receptor Substrate 1 (IRS1))

别名

IRS1

背景

Background: Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors. When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. When bound, IRS1 typically activates phosphatidylinositol 3-kinase p85 subunit. IRS1 interacts with both the NPXY motif of tyrosine-phosphorylated IGF1R and the INSR through the PTB domain. Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Diseases associated with IRS1 include Diabetes Mellitus, Noninsulin-Dependent and Mixed Cell Adenoma. Anti-IRS1pS307 is useful for researchers interested in protein kinase binding, IL-2 pathways, and RET signaling.

基因ID

3667

NCBI登录号

NP_005535

UniProt

P35568

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process