SARS-CoV-2 NSP5 (3CL-Pro) 抗体
3CL-PRO, M-Pro
适用: SARS Coronavirus (SARS-CoV)
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) 抗体
- SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))
- 适用
- SARS Coronavirus (SARS-CoV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SARS-CoV-2 NSP5 (3CL-Pro) antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
Immunogentype:Recombinant - 亚型
- IgG
- Top Product
- Discover our top product 3CL-PRO, M-Pro Primary Antibody
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anti-SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro) (N-Term) antibody
3CL-PRO, M-Pro 适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))
- 别名
- SARS 3Cl Protease (3CL-PRO, M-Pro 产品)
- 别名
- pp1ab antibody, ORF1a polyprotein;ORF1ab polyprotein antibody, ORF1ab antibody
- 物质类
- Viral Protein
- 背景
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Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.
Synonyms: 3CL PRO antibody, 3CLp antibody, nsp5 antibody - 基因ID
- 1489680, 29837498
- UniProt
- P0C6U8
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