Bcl-2 抗体 (N-Term)
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Quick Overview for Bcl-2 抗体 (N-Term) (ABIN126981)
抗原
See all Bcl-2 (BCL2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Detects a 25 kDa protein, corresponding to the apparent molecular weight of the alpha isoform of Bcl-2 on SDS-PAGE immunoblots.
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纯化方法
- Affinity-purified
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免疫原
- Synthetic peptide corresponding to the N-terminus of human Bcl-2
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应用备注
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Western blot analysis (0.5-4 μg/mL). Immunoprecipation (4-8 μg/mL). Immunohistochemistry on frozen sections (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid Repeated freeze/defrost cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. For long-term storage, aliquot and freeze at -20 °C to -70 °C.
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- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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别名
- Bcl-2
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背景
- Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis. Bcl-2 was identified as a gene translocated from chromosome 18 to the immunoglobulin (Ig) heavy chain locus on chromosome 14 in B lymphocytes, a condition that is associated with the onset of human follicular B cell lymphomas. This interchromosomal translocation leads to constitutive expression of Bcl-2, which results in increased survival and expansion of follicular center cell population.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
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基因ID
- 596
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NCBI登录号
- NP_000624
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UniProt
- P10415
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途径
- MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原
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