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Bcl-2 抗体 (N-Term)

The 兔 多克隆 anti-Bcl-2 antibody (ABIN126981) specifically detects Bcl-2 in WB, IP 和 IHC (fro). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN126981
发货至: 中国
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Quick Overview for Bcl-2 抗体 (N-Term) (ABIN126981)

抗原

See all Bcl-2 (BCL2) 抗体
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This Bcl-2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    N-Term

    特异性

    Detects a 25 kDa protein, corresponding to the apparent molecular weight of the alpha isoform of Bcl-2 on SDS-PAGE immunoblots.

    纯化方法

    Affinity-purified

    免疫原

    Synthetic peptide corresponding to the N-terminus of human Bcl-2
  • 应用备注

    Western blot analysis (0.5-4 μg/mL). Immunoprecipation (4-8 μg/mL). Immunohistochemistry on frozen sections (10-20 μg/mL).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid Repeated freeze/defrost cycles.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. For long-term storage, aliquot and freeze at -20 °C to -70 °C.
  • 抗原

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    别名

    Bcl-2

    背景

    Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis. Bcl-2 was identified as a gene translocated from chromosome 18 to the immunoglobulin (Ig) heavy chain locus on chromosome 14 in B lymphocytes, a condition that is associated with the onset of human follicular B cell lymphomas. This interchromosomal translocation leads to constitutive expression of Bcl-2, which results in increased survival and expansion of follicular center cell population.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha

    基因ID

    596

    NCBI登录号

    NP_000624

    UniProt

    P10415

    途径

    MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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