ROCK1 抗体 (Cleavage Site)
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北京 101111
Quick Overview for ROCK1 抗体 (Cleavage Site) (ABIN126943)
抗原
See all ROCK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Cleavage Site
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特异性
- The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.
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产品特性
- Synonyms: ROCK-1, p160ROCK, NY-REN-35, Rho-associated protein kinase 1, Renal carcinoma antigenNY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1
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纯化方法
- Protein G Chromatography.
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免疫原
- Synthetic peptide corresponding to the Caspase-3 cleavage site
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亚型
- IgG1
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anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (N-Term) antibody
KO Validated ROCK1 适用: 人 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1001-1200) antibodyROCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510) antibodyROCK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E2 unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (pSer456), (pThr455) antibodyROCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 400-660) antibodyROCK1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibodyROCK1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 405-668) antibodyROCK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibodyKD Validated ROCK1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB2065 unconjugated Recombinant Antibody
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibodyKD Validated ROCK1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3460 unconjugated Recombinant Antibody
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1094-1323) antibodyROCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1-4 μg/mL. Jurkat cell lysate treated with Camptothecin (2μM for 6-8 hours) can be used as a PositiveControl.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.2 containing 50 % Glycerol, 1 % BSA and 0.02 % Thimerosal.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
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别名
- ROCK1
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背景
- ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.Synonyms: NY-REN-35, ROCK-1, Renal carcinoma antigen NY-REN-35, Rho-associated, Rho-associated protein kinase 1, coiled-coil-containing protein kinase 1, p160ROCK
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基因ID
- 6093
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UniProt
- Q13464
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途径
- Microtubule Dynamics, WNT signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
抗原
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