AKT2 抗体
Quick Overview for AKT2 抗体 (ABIN126856)
抗原
See all AKT2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).
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产品特性
- Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-betaserine/threonine-protein kinase
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纯化方法
- Thiophilic Adsorption and Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Synthetic peptide conjugated to KLH
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亚型
- IgG2a
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibody
AKT2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal X1 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) antibodyAKT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 93-123) antibodyAKT2 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 416-444) antibodyAKT2 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 142-412) antibodyAKT2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (pSer474) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (C-Term) antibodyAKT2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibodyAKT2 适用: 人 WB, FACS, IP 宿主: 小鼠 Monoclonal 8D9 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibodyAKT2 适用: 人, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B6 unconjugated
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应用备注
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Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL, 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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别名
- AKT2 / PKB beta
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背景
- Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
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基因ID
- 208
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UniProt
- P31751
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途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
抗原
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