MAP2K7 抗体
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北京 101111
Quick Overview for MAP2K7 抗体 (ABIN126842)
抗原
See all MAP2K7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes the N-terminus of MKK7.
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产品特性
- Synonyms: MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7,NKK2, MEK7, MKK7, MAP kinase kinase 7, Dual specificity mitogen-activated protein kinasekinase 7
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纯化方法
- Size exclusion chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic peptide conjugated to KLH
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated SW480 cells. ELISA: 0.1 μg/mL. Immunocytochemistry. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
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别名
- MAP2K7
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背景
- The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.Synonyms: Dual specificity mitogen-activated protein kinase kinase 7, JNK-activating kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK7, MKK7, NKK2, c-Jun N-terminal kinase kinase 2
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分子量
- 45 kDa
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基因ID
- 5609
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UniProt
- O14733
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途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
抗原
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