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ERK1 抗体 (pThr-Glu-pTyr)

Name: ERK1 抗体 (pThr-Glu-pTyr)
SKU: ABIN126831
Availability: OutOfStock

The 小鼠单克隆 anti-ERK1 antibody (Clone 12D4) (ABIN126831) specifically detects ERK1 in WB, IF, IP, IHC (fro) 和 EIA. The antibody is reactive with 人, 小鼠, 大鼠和犬 samples.

Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr-Glu-pTyr) antibody (ABIN126831) (ERK1 抗体 (pThr-Glu-pTyr))

1 image

产品编号 ABIN126831

发货至: 中国

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中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for ERK1 抗体 (pThr-Glu-pTyr) (ABIN126831)

抗原

See all ERK1 (MAPK3) 抗体

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

适用

人, 小鼠, 大鼠, 犬

宿主

小鼠

克隆类型

单克隆

标记

This ERK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

12D4

Discover our top product MAPK3 Primary Antibody

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pThr-Glu-pTyr

特异性

This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.

产品特性

Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3

纯化方法

Size exclusion chromatography

组件

incl. positive Control

免疫原

Synthetic phosphopeptide conjugated to KLH

亚型

IgG1
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 101-200) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-100) antibody
MAPK3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibody
MAPK3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E5 unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibody
MAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibody
MAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (N-Term) antibody
Verified MAPK3 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr202), (pTyr204) antibody
MAPK3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr185), (pThr202), (pTyr187), (pTyr204) antibody
MAPK3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C/-80 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

别名

MAPK3 / ERK1

背景

Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK

基因ID

5595

途径

MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins