Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1mg/mL
缓冲液
PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
LEF1
(Lymphoid Enhancer-Binding Factor 1 (LEF1))
别名
LEF1
背景
LEF is a transcription factor of the LEF/TCF protein family. LEF interacts with cytoplasmic beta-catenin and regulates the expression of genes containing a LEF/TCF binding site. The formation of LEF - beta-catenin heterodimers is an important step in the wnt/wg signal transduction pathway.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha