Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN126040)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types: Collagen types 1 and 3: 100 % . Collagen types 4 and 5: 90 %. Collagen type 2: 80 %. Fibronectin, Albumin and Immunoglobulins all < 0.5 %.
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交叉反应 (详细)
- Also reacts to: type II, and V, III, IV
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纯化方法
- Affinity Chromatography on immobilized collagen types I-V
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免疫原
- A mixture of human collagen 1-5 from human placenta.
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应用备注
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Immunohistochemistry on Frozen Sections (1/10-1/40). ELISA (1/2000-1/8000). Immunoflourescence (1/10-1/40).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.5 containing 0.1 % Mannitol and 0.1 % Dextran and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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背景
- Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
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基因ID
- 1277
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NCBI登录号
- NP_000079
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UniProt
- P02452
抗原
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