Neisseria Meningitidis Antigen Orf1 抗体
适用: Neisseria meningitidis
WB
宿主: 兔
Polyclonal
unconjugated
1 image
(1 reference)-
- 抗原
- Neisseria Meningitidis Antigen Orf1
- 适用
- Neisseria meningitidis
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB)
- 特异性
- The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
- 交叉反应 （详细）
- Neisseria meningitidis
- 纯化方法
- Purified by ligand affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
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- 应用备注
- Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
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: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
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- 抗原
- Neisseria Meningitidis Antigen Orf1
- 别名
- Neisseria meningitidis antigen Orf1 / FrpD
- 背景
- Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD
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