TAS1R3
适用: 人, 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 555
应用备注
Western blot. Immunohistochemistry with formalin fixed paraffin embedded tissues: 17 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min, Remove from heat and let stand at room temperature in buffer for 20 min, Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
有效期
12 months
抗原
TAS1R3
(Taste Receptor, Type 1, Member 3 (TAS1R3))
tas1r3 antibody, T1R3 antibody, Sac antibody, T1r3 antibody, taste receptor, type 1, member 3 antibody, taste 1 receptor member 3 antibody, TAS1R3 antibody, tas1r3 antibody, Tas1r3 antibody
背景
The sense of taste provides animals with valuable information about the quality and nutritional value of food. There are four widely accepted categories of taste perception: sweet, bitter, salty and sour. A controversial fifth taste, known as umami or monosodium glutamate (MSG), has also been described. A family of G protein-coupled receptors are involved in taste perception and include T1R, which is involved in sweet and umami taste perception, and T2R, which is involved in bitter taste perception. The T1R family consists of three members: T1R1, T1R2 and T1R3. These proteins form heterodimers, which alter the selectivity of the subunits. The T1R2 and T1R3 heterodimer functions as a receptor for sweet taste, and recognizes several sweet-tasting molecules such as sucrose, saccharin, dulcin and acesulfame-K. The T1R1 and T1R3 heterodimer recognizes L-amino acids to perceive umami taste. Sweet taste transduction is carried out by two pathways. First, sucrose and other sugars activate Gas via the T1Rs, which activates adenylyl cyclase to generate cAMP. TAS1R3 is a Taste Receptor. TAS1R3 combines with TAS1R1 to function as an L-amino-acid receptor, and is only activated by these sweet amino acids.Synonyms: Sweet taste receptor T1R3, TR3, Taste receptor type 1 member 3