This antibody recognises EMR2, a member of the epidermal growth factor-seven transmembrane (EGFTM7) family of proteins, which is closely related to CD97. Clone 2A1 specifically recognises the stalk region of EMR2.
纯化方法
Affinity Chromatography on Protein G.
免疫原
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Flow Cytometry: Use 10 μL of 1/50-1/200 diluetd antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Western Blot. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.Synonyms: EGF-like module EMR2, EGF-like module-containing mucin-like hormone receptor-like 2