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- 抗原 See all Cyclin B1 (CCNB1) 抗体
- Cyclin B1 (CCNB1)
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抗原表位
- AA 120-131, pSer126
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Cyclin B1 antibody is un-conjugated
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应用范围
- Enzyme Immunoassay (EIA)
- 特异性
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91 % homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83 % homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.
- Top Product
- Discover our top product CCNB1 Primary Antibody
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anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated
anti-Cyclin B1 (CCNB1) (C-Term) antibodyCCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 271-433) antibodyCCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-90) antibodyCCNB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-Cyclin B1 (CCNB1) (Internal Region) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (pSer126) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKO Validated CCNB1 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) andWestern blot (1: 100-1: 1,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approx. 48 kDa in size corresponding to Cyclin B1 by western blotting inthe appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.6 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 2-8 °C.
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- 抗原
- Cyclin B1 (CCNB1)
- 别名
- Cyclin B1 (CCNB1 产品)
- 别名
- ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody
- 背景
- Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1
- 基因ID
- 891
- NCBI登录号
- NP_114172
- UniProt
- P14635
- 途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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