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- 抗原 See all CBX4 抗体
- CBX4 (Chromobox Homolog 4 (CBX4))
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抗原表位
- AA 95-107
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CBX4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein. Cross reactivity with PC2 protein from mouse may occur as a 92 % homology (BLAST) is noted for the immunized sequence. Cross reactivity with PC2 protein from other sources is not known.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.
- Top Product
- Discover our top product CBX4 Primary Antibody
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anti-Chromobox Homolog 4 (CBX4) (AA 397-514) antibody
CBX4 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 6C5G3 unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 1-160) antibodyCBX4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 401-500) antibodyCBX4 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 401-560) antibodyCBX4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 1-290) antibodyCBX4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 401-560) antibodyCBX4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 80-110), (N-Term) antibodyCBX4 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2931 unconjugated
anti-Chromobox Homolog 4 (CBX4) (N-Term) antibodyCBX4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 150-200) antibodyCBX4 适用: 人, 小鼠, 犬, Cow, 豚鼠, 兔, Pig IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 53-102) antibodyCBX4 适用: 人, 小鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This affinity purified antibody has been tested for use in immunofluorescence staining ofcultured cells and western blotting using transfected cell lysates. The antibody is alsofunctional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for westernblotting represent a starting point dilution and further optimization may be required. Theantibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure thatis termed the PcG body, corresponding to the known localization of PC2. The antibody hasbeen successfully used to detect FLAG-tagged transfected hPC2 (see figure below). Itdetects a weak band that probably corresponds to endogenous PC2, however, a strongsecondary band is also seen at 50 kD in all cell lines thus far tested. This suggests that theantibody may also react with another highly expressed ubiquitous protein.
- 限制
- 仅限研究用
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- 浓度
- 0.8 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 2-8 °C.
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- 抗原
- CBX4 (Chromobox Homolog 4 (CBX4))
- 别名
- CBX4 / PC2 (CBX4 产品)
- 别名
- MPc2 antibody, PC2 antibody, NBP16 antibody, XPolycomb antibody, cbx4-a antibody, im:6911625 antibody, zgc:158588 antibody, chromobox 4 antibody, chromobox 4 S homeolog antibody, chromobox homolog 4 antibody, chromobox homolog 4 (Pc class homolog, Drosophila) antibody, CBX4 antibody, Cbx4 antibody, cbx4.S antibody, cbx4 antibody
- 背景
- PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.Synonyms: Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, Polycomb 2 homolog
- 基因ID
- 8535
- NCBI登录号
- NP_003646
- UniProt
- O00257
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