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Huntingtin 抗体 (pSer421)

Name: Huntingtin 抗体 (pSer421)
SKU: ABIN117993
Availability: OutOfStock

HTT 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated

Western blot analysis is shown using anti-Huntingtin pS421 antibody to detect endogenous protein present in an unstimulated human PC-3 whole cell lysate (arrowhead). (Huntingtin 抗体 (pSer421))

1 image

产品编号 ABIN117993

发货至: 中国

Datasheet as PDF

抗原 See all Huntingtin (HTT) 抗体

Huntingtin (HTT)
抗原表位
15

6

4

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 416-424, pSer421
适用
64

46

37

6

3

2

2

1

1

1

1

1

1

1

1

1

1
人
宿主
61

21

2
兔
Compatible Secondaries
克隆类型
54

29
多克隆
标记
56

3

3

3

3

3

3

3

1

1

1

1

1

1

1
This Huntingtin antibody is un-conjugated
应用范围
41

33

25

14

14

13

13

9

7

4

4

2

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

序列

Human Huntingtin protein, 3144 aa, predicted MW 347.7 kDa

特异性

This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntingtin protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntingtin homologues from chimpanzee, pig and chicken. Cross reactivity with Huntingtin protein homologues from mouse and rat may also occur as sequence homology varies by one amino acids residues in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

纯化方法

Immunoaffinity chromatography.

免疫原

Synthetic peptide corresponding aa 416-424 of Human huntingtin protein.

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应用备注

Western blotting (1: 500 - 1: 3,000). ELISA (1: 10,000 - 1: 40,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.8 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 2-8 °C.
抗原

Huntingtin (HTT)

别名

Huntingtin (HTT 产品)

别名

HTT antibody, hd antibody, ZHD antibody, CG9995 antibody, Dmel\\CG9995 antibody, HD antibody, Hsap\\HD antibody, Htt antibody, dHtt antibody, dhtt antibody, SLC6A4 antibody, huntington antibody, it15 antibody, htt antibody, IT15 antibody, AI256365 antibody, C430023I11Rik antibody, Hd antibody, Hdh antibody, huntingtin antibody, HTT antibody, htt antibody, LOC373520 antibody, Htt antibody

背景

Huntingtin (also known as Huntington's disease protein and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.Synonyms: HD, HTT, Huntington Disease Protein, IT15

基因ID

3064

NCBI登录号

NP_002102

UniProt

P42858

途径

PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport