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NF-kB p65 抗体 (N-Term, NLS Domain)

Name: NF-kB p65 抗体 (N-Term, NLS Domain)
SKU: ABIN117926
Availability: OutOfStock

This anti-NF-kB p65 antibody is a 兔多克隆 antibody detecting NF-kB p65 in WB, IF, EIA 和 EMSA. Suitable for 人.

Immunofluorescencechemistry. (NF-kB p65 抗体 (N-Term, NLS Domain))

Immunohistochemistry. (NF-kB p65 抗体 (N-Term, NLS Domain))

IHC (p)

2 images

产品编号 ABIN117926

发货至: 中国

Datasheet as PDF

Quick Overview for NF-kB p65 抗体 (N-Term, NLS Domain) (ABIN117926)

抗原

See all NF-kB p65 (NFkBP65) 抗体

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

适用

人

宿主

兔

克隆类型

多克隆

标记

This NF-kB p65 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Discover our top product NFkBP65 Primary Antibody

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N-Term, NLS Domain

序列

Peptide Sequence: R-I-E-E-K-R-K-R-T-Y-E-T

特异性

This affinity purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFκB heterodimer. Therefore, the antibody selectively binds to the activated form of NFκB. Anti-NFκB p65 NLS may react non-specifically with other proteins.

纯化方法

Affinity Purified

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkappaB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
anti-Nuclear Factor-kB p65 (NFkBP65) (AA 51-100) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (Center) antibody
NFkBP65 适用: 人, 小鼠 WB, IF, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 19-306) antibody
NFkBP65 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys314), (acLys315) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 50-180) antibody
NFkBP65 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Suitable for Immunohistochemistry, Immunoblotting, ELISA and supershift assays. Thisproduct was assayed by Immunoblot and found to be reactive against NFκB p65 NLS at adilution of 1: 2,000 followed by reaction with Peroxidase conjugated Affinity Purified

限制

仅限研究用
浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

储存条件

-20 °C

储存方法

Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.
抗原

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

别名

RELA / NF-kB p65

背景

NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. IκB- α binding masks the NFκB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFκB and set off signalling cascades that ultimately converge on the IκB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IκB-α and this phophorylation event targets IκB-α for degradation. As a consequence, NFκB NLS is uncovered and nuclear translocation occurs.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65

基因ID

5970

NCBI登录号

NP_001138610

UniProt

Q04206

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins