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Bcl-2 抗体 (pSer70) (Alexa Fluor 647)

Name: Bcl-2 抗体 (pSer70) (Alexa Fluor 647)
SKU: ABIN1177234
Availability: OutOfStock

This anti-Bcl-2 antibody is a 小鼠单克隆 antibody detecting Bcl-2 in ICS. Suitable for 人. This Primary Antibody has been cited in 5+ publications.

(5 references)

产品编号 ABIN1177234

发货至: 中国

Quick Overview for Bcl-2 抗体 (pSer70) (Alexa Fluor 647) (ABIN1177234)

抗原

See all Bcl-2 (BCL2) 抗体

Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Bcl-2 antibody is conjugated to Alexa Fluor 647

应用范围

Intracellular Staining (ICS)

克隆位点

N46-467

Discover our top product BCL2 Primary Antibody

抗原表位
55

36

26

23

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21

16

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16

12

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8

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4

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3

3

2

2

2

2

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2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer70

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Peptide

亚型

IgG1
anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody
BCL2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 101-160) antibody
BCL2 适用: 人, 小鼠, 大鼠, 兔, Cow, 小鸡, Bacteria WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibody
BCL2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibody
BCL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody
BCL2 适用: 人 WB, IHC, FACS, IF, StM, ISt 宿主: 小鼠 Monoclonal 100-D5 unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) antibody
BCL2 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal BCL2-2210R unconjugated Recombinant Antibody

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody
BCL2 适用: 人 WB, FACS, IP, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bcl-2-100 unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PE)
BCL2 适用: 人 ICFC 宿主: 小鼠 Monoclonal Bcl-2-100 PE

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 118-165) antibody
BCL2 适用: 人 WB, FACS, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 1-211) antibody
BCL2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

样本量

5 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Geng, Tang, Li, Yang, Choi, Kazim, Zhang: "Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, (2011) (PubMed).

Sarkar, Korolchuk, Renna, Imarisio, Fleming, Williams, Garcia-Arencibia, Rose, Luo, Underwood, Kroemer, OKane, Rubinsztein: "Complex inhibitory effects of nitric oxide on autophagy." in: Molecular cell, Vol. 43, Issue 1, pp. 19-32, (2011) (PubMed).

Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, (2009) (PubMed).

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, (2001) (PubMed).

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, (1998) (PubMed).
抗原

Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

别名

Bcl-2

背景

The N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50

途径

MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling