LCP2 抗体 (pTyr128) (Alexa Fluor 647)
(3 references)-
- 抗原 See all LCP2 抗体
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
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抗原表位
- pTyr128
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LCP2 antibody is conjugated to Alexa Fluor 647
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human SLP-76
- 克隆位点
- J141-668-36-58
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product LCP2 Primary Antibody
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anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 202-463) antibody
LCP2 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibodyVerified LCP2 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibodyLCP2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 284-533) antibodyLCP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 94-143), (pTyr128) antibodyLCP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (pTyr128) antibodyLCP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 46-80) antibodyLCP2 适用: 人 WB 宿主: 兔 Polyclonal RB56244 unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 303-337) antibodyLCP2 适用: 人 WB 宿主: 兔 Polyclonal RB56245 unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibodyLCP2 适用: 人 WB, IP, FACS 宿主: 小鼠 Monoclonal H76 unconjugated
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibodyLCP2 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer, Cat. No. 558049) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer, Cat. No. 554655). Any of the 3 BD Phosflow™ Perm Buffers may be used.
- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).
: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).
: "Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 3769-73, (1996) (PubMed).
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: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).
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- 抗原
- LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))
- 别名
- SLP-76 (LCP2 产品)
- 别名
- AI323664 antibody, BB161688 antibody, SLP-76 antibody, SLP76 antibody, twm antibody, Slp76 antibody, lymphocyte cytosolic protein 2 antibody, Lcp2 antibody, LCP2 antibody
- 背景
- SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR) -mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 128 (Y128) brings the Rho family guanine nucleotide exchange factor Vav1 and the Nck adapter protein, which binds to p21-activated kinase (PAK1) and Wiskott-Aldrich syndrome protein (WASP), into the activation complex. Vav1, PAK1, and WASP may mediate TCR-stimulated actin cytoskeletal rearrangement. The J141-688.36.58 monoclonal antibody recognizes the phosphorylated Y128 of activated SLP-76.
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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