(9 references)-
- 抗原 See all CD14 抗体
- CD14
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD14 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Mouse monocyte/macrophage cell line
- 克隆位点
- 4C1-CD14
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD14 Primary Antibody
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anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated
anti-CD14 (CD14) (AA 20-214) antibodyCD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated
anti-CD14 (CD14) (AA 20-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 14G1F3 unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated
anti-CD14 (CD14) (AA 230-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated
anti-CD14 (CD14) (AA 54-83), (N-Term) antibodyCD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated
anti-CD14 (CD14) (AA 201-300) antibodyCD14 适用: 人, 小鼠, 大鼠, 犬, 绵羊, 兔 WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2397 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人, Non-Human Primate FACS, WB, ELISA, IP 宿主: 小鼠 Monoclonal MEM-18 unconjugated
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- 应用备注
- For western blot and flow cytometric analysis, investigators may find Rat Anti-Mouse CD14 mAb rmC5-3 (Cat. No. 553738), FITC Rat Anti-Mouse CD14 (Cat. No. 553739) and/or PE Rat Anti-Mouse CD14 (Cat. No. 553740) to be useful.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Effect of CD14 blockade on endotoxin-induced acute lung injury in mice." in: American journal of respiratory cell and molecular biology, Vol. 29, Issue 2, pp. 252-8, (2003) (PubMed).
: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).
: "Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster." in: Trends in immunology, Vol. 23, Issue 6, pp. 301-4, (2002) (PubMed).
: "Cathelicidin family of antibacterial peptides CAP18 and CAP11 inhibit the expression of TNF-alpha by blocking the binding of LPS to CD14(+) cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3329-38, (2001) (PubMed).
: "Micrococcus luteus teichuronic acids activate human and murine monocytic cells in a CD14- and toll-like receptor 4-dependent manner." in: Infection and immunity, Vol. 69, Issue 4, pp. 2025-30, (2001) (PubMed).
: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, (2001) (PubMed).
: "Heterogeneity of human blood monocytes: the CD14+ CD16+ subpopulation." in: Immunology today, Vol. 17, Issue 9, pp. 424-8, (1997) (PubMed).
: "Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice." in: Immunity, Vol. 4, Issue 4, pp. 407-14, (1996) (PubMed).
: "Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide." in: The Journal of experimental medicine, Vol. 179, Issue 5, pp. 1671-6, (1994) (PubMed).
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: "Effect of CD14 blockade on endotoxin-induced acute lung injury in mice." in: American journal of respiratory cell and molecular biology, Vol. 29, Issue 2, pp. 252-8, (2003) (PubMed).
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- 抗原
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
- 背景
- The 4C1/CD14 antibody reacts with CD14, a ~55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein. Flow cytometric analysis demonstrates that 4C1/CD14 antibody stains CD14 transfectants, the monocyte/macrophage cell line RAW 264.7 peritoneal exudate neutrophils and macrophages, and cultured bone marrow-derived macrophages, but not undifferentiated bone marrow cells, splenocytes, or peripheral blood leukocytes. This staining pattern is similar to that of the alternate anti-mouse CD14 mAb rmC5-3, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils. Therefore, data suggest that CD14 expression by leukocyte populations may differ in mice and humans. CD14 is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein). After binding to LPS-LPB, CD14 associates with integral membrane proteins (such as TLR4-MD-2) which mediate signal transduction. It is involved in the development of endotoxic shock, innate immunity, inflammatory responses, and LPS-stimulated bone resorption. Unlike mAb rmC5-3, the 4C1/CD14 antibody blocks the binding of LPS to CD14.
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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