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CTLA4 抗体

Name: CTLA4 抗体
SKU: ABIN1176976
Availability: OutOfStock

This anti-CTLA4 antibody is a Armenian Hamster 单克隆 antibody detecting CTLA4 in FACS, IP 和 BR. Suitable for 小鼠. This Primary Antibody has been cited in 18+ publications.

(18 references)

产品编号 ABIN1176976

发货至: 中国

Quick Overview for CTLA4 抗体 (ABIN1176976)

抗原

See all CTLA4 抗体

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

适用

小鼠

宿主

Armenian Hamster

克隆类型

单克隆

标记

This CTLA4 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

克隆位点

UC10-4F10

Discover our top product CTLA4 Primary Antibody

品牌

BD Pharmingen™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

过滤

0.2 μm filtered

内毒素水平

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

免疫原

Mouse CTLA-4 IgG2a Fusion

亚型

IgG1 kappa
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-135) antibody
CTLA4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F1 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody (PE)
CTLA4 适用: 人 FACS 宿主: 小鼠 Monoclonal BNI3 PE

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-162) antibody
CTLA4 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9D2E9 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody
CTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody
CTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibody
CTLA4 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM50 unconjugated Recombinant Antibody

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibody
CTLA4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8B3F8 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 75-170) antibody
CTLA4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 41-90) antibody
CTLA4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 52-211) antibody
CTLA4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Since CD152 is expressed at low density on activated T cells, it may be necessary to amplify the signal by using a biotinylated second-step reagent, followed by a bright third-step reagent. We have found that biotin-conjugated mouse anti-hamster IgG (Cat. No. 554010) plus Streptavidin-PE (Cat. No. 554061) are effective. BD Mouse Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) may help to reduce non-specific binding of the antibody to cells bearing Fcgamma receptors. Since a large proportion of the CTLA-4 molecule is intracellular, detection of the antigen is enhanced by staining cells permeabilized with the BD Cytofix/Cytoperm™ intracellular staining kit (Cat. No. 554714).

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

储存液

Azide free

储存条件

4 °C

储存方法

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, (2000) (PubMed).

Lee, Chuang, Griffin, Khattri, Hong, Zhang, Straus, Samelson, Thompson, Bluestone: "Molecular basis of T cell inactivation by CTLA-4." in: Science (New York, N.Y.), Vol. 282, Issue 5397, pp. 2263-6, (1999) (PubMed).

Issazadeh, Zhang, Sayegh, Khoury: "Acquired thymic tolerance: role of CTLA4 in the initiation and maintenance of tolerance in a clinically relevant autoimmune disease model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 761-5, (1999) (PubMed).

Cilio, Daws, Malashicheva, Sentman, Holmberg: "Cytotoxic T lymphocyte antigen 4 is induced in the thymus upon in vivo activation and its blockade prevents anti-CD3-mediated depletion of thymocytes." in: The Journal of experimental medicine, Vol. 188, Issue 7, pp. 1239-46, (1998) (PubMed).

Waterhouse, Bachmann, Penninger, Ohashi, Mak: "Normal thymic selection, normal viability and decreased lymphoproliferation in T cell receptor-transgenic CTLA-4-deficient mice." in: European journal of immunology, Vol. 27, Issue 8, pp. 1887-92, (1997) (PubMed).

McCoy, Camberis, Gros: "Protective immunity to nematode infection is induced by CTLA-4 blockade." in: The Journal of experimental medicine, Vol. 186, Issue 2, pp. 183-7, (1997) (PubMed).

Tivol, Boyd, McKeon, Borriello, Nickerson, Strom, Sharpe: "CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5091-4, (1997) (PubMed).

Alegre, Noel, Eisfelder, Chuang, Clark, Reiner, Thompson: "Regulation of surface and intracellular expression of CTLA4 on mouse T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 11, pp. 4762-70, (1996) (PubMed).

Linsley, Bradshaw, Greene, Peach, Bennett, Mittler: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, (1996) (PubMed).

Marengère, Waterhouse, Duncan, Mittrücker, Feng, Mak: "Regulation of T cell receptor signaling by tyrosine phosphatase SYP association with CTLA-4." in: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1170-3, (1996) (PubMed).

Perkins, Wang, Donovan, He, Mark, Guan, Wang, Walunas, Bluestone, Listman, Finn: "Regulation of CTLA-4 expression during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4154-9, (1996) (PubMed).

Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, (1996) (PubMed).

Kearney, Walunas, Karr, Morton, Loh, Bluestone, Jenkins: "Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1032-6, (1995) (PubMed).

Tivol, Borriello, Schweitzer, Lynch, Bluestone, Sharpe: "Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4." in: Immunity, Vol. 3, Issue 5, pp. 541-7, (1995) (PubMed).

Walunas, Lenschow, Bakker, Linsley, Freeman, Green, Thompson, Bluestone: "CTLA-4 can function as a negative regulator of T cell activation." in: Immunity, Vol. 1, Issue 5, pp. 405-13, (1995) (PubMed).

Waterhouse, Penninger, Timms, Wakeham, Shahinian, Lee, Thompson, Griesser, Mak: "Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4." in: Science (New York, N.Y.), Vol. 270, Issue 5238, pp. 985-8, (1995) (PubMed).

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, (1994) (PubMed).
抗原

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

别名

CD152

背景

The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.
Synonyms: CTLA-4

途径

Cancer Immune Checkpoints