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CTLA4 抗体

Name: CTLA4 抗体
SKU: ABIN1176976
Availability: OutOfStock

This Armenian Hamster 单克隆 antibody specifically detects CTLA4 in FACS, IP 和 BR. It exhibits reactivity toward 小鼠. It has been mentioned in 18+ publications

(18 references)

产品编号 ABIN1176976

发货至: 中国

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Quick Overview for CTLA4 抗体 (ABIN1176976)

抗原

See all CTLA4 抗体

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

适用

小鼠

宿主

Armenian Hamster

克隆类型

单克隆

标记

This CTLA4 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

克隆位点

UC10-4F10

Discover our top product CTLA4 Primary Antibody

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

过滤

0.2 μm filtered

内毒素水平

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

免疫原

Mouse CTLA-4 IgG2a Fusion

亚型

IgG1 kappa
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-135) antibody
CTLA4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F1 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody (PE)
CTLA4 适用: 人 FACS 宿主: 小鼠 Monoclonal BNI3 PE

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-162) antibody
CTLA4 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 9D2E9 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody
CTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody
CTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibody
CTLA4 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM50 unconjugated Recombinant Antibody

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibody
CTLA4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8B3F8 unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 75-170) antibody
CTLA4 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody
CTLA4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 52-211) antibody
CTLA4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Since CD152 is expressed at low density on activated T cells, it may be necessary to amplify the signal by using a biotinylated second-step reagent, followed by a bright third-step reagent. We have found that biotin-conjugated mouse anti-hamster IgG (Cat. No. 554010) plus Streptavidin-PE (Cat. No. 554061) are effective. BD Mouse Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) may help to reduce non-specific binding of the antibody to cells bearing Fcgamma receptors. Since a large proportion of the CTLA-4 molecule is intracellular, detection of the antigen is enhanced by staining cells permeabilized with the BD Cytofix/Cytoperm™ intracellular staining kit (Cat. No. 554714).

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

储存液

Azide free

储存条件

4 °C

储存方法

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, (2000) (PubMed).

Lee, Chuang, Griffin, Khattri, Hong, Zhang, Straus, Samelson, Thompson, Bluestone: "Molecular basis of T cell inactivation by CTLA-4." in: Science (New York, N.Y.), Vol. 282, Issue 5397, pp. 2263-6, (1999) (PubMed).

Issazadeh, Zhang, Sayegh, Khoury: "Acquired thymic tolerance: role of CTLA4 in the initiation and maintenance of tolerance in a clinically relevant autoimmune disease model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 761-5, (1999) (PubMed).

Cilio, Daws, Malashicheva, Sentman, Holmberg: "Cytotoxic T lymphocyte antigen 4 is induced in the thymus upon in vivo activation and its blockade prevents anti-CD3-mediated depletion of thymocytes." in: The Journal of experimental medicine, Vol. 188, Issue 7, pp. 1239-46, (1998) (PubMed).

Waterhouse, Bachmann, Penninger, Ohashi, Mak: "Normal thymic selection, normal viability and decreased lymphoproliferation in T cell receptor-transgenic CTLA-4-deficient mice." in: European journal of immunology, Vol. 27, Issue 8, pp. 1887-92, (1997) (PubMed).

McCoy, Camberis, Gros: "Protective immunity to nematode infection is induced by CTLA-4 blockade." in: The Journal of experimental medicine, Vol. 186, Issue 2, pp. 183-7, (1997) (PubMed).

Tivol, Boyd, McKeon, Borriello, Nickerson, Strom, Sharpe: "CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5091-4, (1997) (PubMed).

Alegre, Noel, Eisfelder, Chuang, Clark, Reiner, Thompson: "Regulation of surface and intracellular expression of CTLA4 on mouse T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 11, pp. 4762-70, (1996) (PubMed).

Linsley, Bradshaw, Greene, Peach, Bennett, Mittler: "Intracellular trafficking of CTLA-4 and focal localization towards sites of TCR engagement." in: Immunity, Vol. 4, Issue 6, pp. 535-43, (1996) (PubMed).

Marengère, Waterhouse, Duncan, Mittrücker, Feng, Mak: "Regulation of T cell receptor signaling by tyrosine phosphatase SYP association with CTLA-4." in: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1170-3, (1996) (PubMed).

Perkins, Wang, Donovan, He, Mark, Guan, Wang, Walunas, Bluestone, Listman, Finn: "Regulation of CTLA-4 expression during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4154-9, (1996) (PubMed).

Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, (1996) (PubMed).

Kearney, Walunas, Karr, Morton, Loh, Bluestone, Jenkins: "Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1032-6, (1995) (PubMed).

Tivol, Borriello, Schweitzer, Lynch, Bluestone, Sharpe: "Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4." in: Immunity, Vol. 3, Issue 5, pp. 541-7, (1995) (PubMed).

Walunas, Lenschow, Bakker, Linsley, Freeman, Green, Thompson, Bluestone: "CTLA-4 can function as a negative regulator of T cell activation." in: Immunity, Vol. 1, Issue 5, pp. 405-13, (1995) (PubMed).

Waterhouse, Penninger, Timms, Wakeham, Shahinian, Lee, Thompson, Griesser, Mak: "Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4." in: Science (New York, N.Y.), Vol. 270, Issue 5238, pp. 985-8, (1995) (PubMed).

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, (1994) (PubMed).
抗原

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

别名

CD152

背景

The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.
Synonyms: CTLA-4

途径

Cancer Immune Checkpoints