This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.
产品特性
Absorption / Emission: 495 nm / 528 nm Molar Ratio: 3.0 moles DTAF per mole of Rabbit IgG
Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 2.0 mL of deionized water (or equivalent).
浓度
10.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD