Ubiquitin 抗体
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- 抗原 See all Ubiquitin 抗体
- Ubiquitin
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适用
- Saccharomyces cerevisiae, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ubiquitin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This purified polyclonal antibody reacts with Ubiquitin by ELISA and Western blot.
- 纯化方法
- Multi-step process.
- 免疫原
- This purified antibody was prepared from Rabbit serum after repeated immunizations with Ubiquitin coupled to Rabbit IgG using glutaraldehyde.
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, 马 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (APC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC
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- 应用备注
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Suitable for ELISA (1/1,000-1/5,000) and Western blot (1/200-1/1,000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. For detection of free Ub by Western blotting use Tris-Tricine SDS-PAGE and autoclavednitrocellulose filters after the transfer and before blocking and addition of anti-Ubantibodies. Details on Western blotting procedures are found in Mimnaugh et al. , (1999 and 2002).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- Ubiquitin
- 别名
- Ubiquitin (Ubiquitin 产品)
- 别名
- CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
- 背景
- Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the ε-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates. Multi-ubiquitin chains can be built up on a single lysine of the target protein, by isopeptide bond formation usually between the carboxyl group of Gly76 of one Ub with the amino group of the side chain of Lys of the preceding ubiquitin. Although Lys48 of Ub is most frequently used for multi-ubiquitin chain formation, other lysines have also been shown to form bonds with Gly76. If a chain of multiple copies of ubiquitin is attached to a protein, this appears to target the protein for degradation by the large intacellular protease known as the 26S proteasome. The process of intracellular proteasomal proteolysis is very rapid and efficient. This makes detection of certain ubiquitinated proteins extremely difficult. Several specific inhibitors of proteasomes are commercially available and have been proven to be very useful in research applications. The detection of ubiquitinated species is further complicated by the fact that the process of ubiquitination is reversible and is accomplished by highly specific proteases present in all eukaryotic cells. It is therefore recommended that cell lysates are kept on ice and the lysis buffers contain one or more of cysteine protease inhibitors (n-ethylmaleimide, E64, etc.).Synonyms: Polyubiquitin-B, UBB
- 基因ID
- 7314
- UniProt
- P0CG47
- 途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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