Caspase 4/11 p20 (AA 81-373) 抗体 (Biotin)
适用: 人, 小鼠
IP, WB
宿主: 小鼠
Monoclonal
Flamy-1
Biotin
-
- 抗原
- Caspase 4/11 p20
- 抗原表位
- AA 81-373
- 适用
- 人, 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- Biotin
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 特异性
- Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11.
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified from concentrated hybridoma tissue culture supernatant.
- 纯度
- >95 % (SDS-PAGE)
- 免疫原
- Recombinant mouse Caspase-11 (aa 81-373).
- 克隆位点
- Flamy-1
- 亚型
- IgG2b
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - 有效期
- 12 months
-
- 抗原
- Caspase 4/11 p20
- 背景
- Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli. Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.
- UniProt
- P70343
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